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. 2017 Jun 2;12:4195–4208. doi: 10.2147/IJN.S132190

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© 2017 Song et al. This work is published and licensed by Dove Medical Press Limited

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Transfection efficiency of PDAPEI to SCs.

Notes: (A) The naked miR-221/222 group, (B) PEI25kDa/miR-221/222 group (at N/P ratio of 10), (C) PDAPEI/miR-221/222 group (at N/P ratio of 200), (D) The percentage of EGFP-positive cells (%) from different groups, (E) Transfection efficiency of PDAPEI and PEI25kDa at different N/P. Transfection efficiency was measured by flow cytometry (A1–C1, observed by light microscopy; A2–C2, observed by fluorescence microscopy; Scale bar, 100 μm. *P<0.05, PDAPEI/miR-221/222 group versus PEI25kDa/miR-221/222 group; ^ϕϕP<0.01, PEI25kDa/miR-221/222 group versus naked miR-221/222 group; ^###P<0.001, PDAPEI/miR-221/222 group versus naked miR-221/222 group).

Abbreviations: PDAPEI, PEI (1.8 kDa, branched) derivative with 2,6-pyridinedicarboxaldehyde (PDA) linkage; PEI, polyethylenimine; SCs, Schwann cells.