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Co-immunoprecipitation (IP) assays to detect association of endogenous LSD1, CoREST and Pit-1 with RIP140 antibody in retinoic acid (RA)-treated embryonic stem cells (ESCs) (A) and RA-treated embryoid bodies (B left panel). (B right panel) Co-IP assays to detect association of endogenous CoREST, RIP140, and Pit-1 with LSD1 antibody in RA-treated ESCs. (C): Re-ChIP detection of RIP140-LSD1 and RIP140-Pit-1 complex on Pax6 P1 in ESC treated with RA (3 days).
