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. Author manuscript; available in PMC: 2017 Jun 16.
Published in final edited form as: Stem Cells. 2015 Oct 9;34(1):114–123. doi: 10.1002/stem.2190

Figure 4.

Figure 4

RIP140 directly interacts with LSD1 to regulate its activity. (A): Pull-down assay for HA-LSD1 full-length (FL) interaction with Flag-RIP140 (FL), Flag-RD1, Flag-RD1-2, Flag-RD2-3, or Flag-RD3-4. Positive interaction denoted by *. (B): Pull-down assay for Flag-RIP140 (FL) interaction with HA-LSD1 (FL) or LSD1 truncated proteins. Positive interaction denoted by *. (C): In vitro LSD1 demethylase activity detected using free bulk histones as substrates and bacteria-expressed LSD1 and RIP140 (lower panel), analyzed by Western blotting using anti-H3k4me2 (upper) or anti-H3k4me1 (middle). Controls include antibody detected H3 (H3 panel).