Figure 3. CARMIL2-deficiency impairs CD28-mediated T-cell differentiation.

(a) Contour plots of CD25⁺CD127^low and CD25⁺FOXP3⁺ T_reg and summary of T_reg percentages for six HD (open squares), P1.1 (black circle), P1.2 (black rhomb), P2.1 (black up-pointing triangle) and P2.2 (black down-pointing triangle). (b) Contour plots of CD4 and CD8 naive (T_N, CD45R0⁻CD27⁺), central memory (T_CM, CD45R0⁺CD27⁺), effector memory (T_EM, CD45R0⁺CD27⁻) and effector (T_EFF, CD45R0⁻CD27⁻) T-cells (corresponding percentages are indicated in each square) and summary of CD4 and CD8 T-cell subtype percentages for six HD and four patients. Small horizontal lines indicate the median. Each symbol represents an individual donor. Data are representative for four independent experiments with n=2. Significance levels are calculated with Welch's t-test and indicated in the summary graphs (NS=non significant).