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. 2017 Jan 23;8:14209. doi: 10.1038/ncomms14209

Figure 7. CARMIL2-deficiency impairs CD28 co-signalling.

Figure 7

(a) Representative immunoblots for pZAP70, pERK1/2, pPKCθ and pNF-κB (p65) with total protein obtained from HD1, HD2, P2.1 and P2.2 T lymphoblasts stimulated with anti-CD3, anti-CD3/CD28 and P/I for 0, 2, 5, 10, 15 and 30 and 5 min, respectively. Actin serves as loading control, molecular weight markers are indicated in kDa and asterisks mark non-specific bands. Data are representative of two independent experiments. (b) Median of pZAP70, pERK1/2, pPKCθ, pNF-κB (p65) and IkBa in CD4 T cells of T lymphoblasts from HD1 and HD2 (open squares), P1.1 (black circle) and P2.2 (black down-pointing triangle) stimulated with anti-CD3 and anti-CD3/CD28 for 0, 2, 5, 15 and 30 min by flow cytometry. (c) Summary of median (phospho-) protein levels for two HD and two patients at the indicated time points with n=3. Significance levels are calculated with Welch's t-test (NS=non significant).