Figure 2. AG influences multiple pathways involved in central metabolism, respiration and redox balance in Mtb.
Heat maps depicting expression of genes (log2fold-change; p≤0.05) coordinating respiration, CCM, iron-metabolism and redox balance for untreated and 6 hr of AG-treated Mtb from two biological samples.
Figure 2—figure supplement 1. qRT-PCR analysis of Mtb exposed to different concentrations of AG for indicated time points.
Fold change for each transcript was measured with respect to untreated wt Mtb by normalizing expression with the 16srRNA transcript. Error bars represent standard deviations from mean. Data are representative of at least two independent experiments done in duplicate.
Figure 2—figure supplement 2. Comparative analysis of genes differentially regulated by AG treatment and upon depletion of mycothiol or ergothioneine buffers.
Heat maps (absolute fold change, p≤0.05) of genes differentially regulated in response to AG treatment and their status in (A) ergothioneine (ΔegtA) and (B) mycothiol (ΔmshA) mutant strains of Mtb. The expression data of MtbΔegtA and MtbΔmshA strains were obtained from a recently published study (Saini et al., 2016).
Figure 2—figure supplement 3. Overlapping regulation of genes in response to AG and oxidative stress.
AG and cumene hydroperoxide (CHP; oxidant) response network was prepared as described in Materials and methods. The vein diagram representing nodes present in top 1% network of Mtb under AG stress and oxidative stress.
Figure 2—figure supplement 4. Heat maps depicting gene expression profile (log2fold-change) of Mtb untreated or treated with 1X and 5X MIC of AG for 6 and 12 hr from at least three biological samples.
