Figure 3. AG influences the internal redox physiology of Mtb.
Wt Mtb or M. bovis BCG was grown to OD600 of 0.4 and treated with 10X MIC of AG). At indicated time points, cells were analyzed for (A) NADH/NAD+ estimation, (B) ROS measurement using oxidant-sensitive fluorescent dye; 2',7'-dichlorofluorescein diacetate (DCFDA), and (C) Superoxide estimation using dihydroethidium (DHE) as described in Materials and methods. (D) Wt Mtb was grown as described earlier and exposed to specified concentrations of Amox in the presence of 8 µg/ml of Clav for 10 days in the presence or absence of thiourea and survival was measured using colony-forming unit (CFU) counts. Error bars represent standard deviations from the mean. *p≤0.05, *p≤0.01 and ***p≤0.001. Data are representative of at least two independent experiments done in duplicate.