Figure 5. Mycothiol mediates tolerance to AG.
Wt Msm and MsmΔmshA strains were grown to OD600 of 0.4 and either treated with various concentrations of (A) Amox and Clav (8 µg/ml) or (b) Clav and Amox (10 µg/ml) and % inhibition in growth was measured by Alamar blue (AB) assay as described in Materials and methods. (C) Wt Mtb, MtbΔmshA, mshA-comp and mshA:OE strains were exposed to 10X MIC of AG and survival was monitored by measuring CFU over time. Error bars represent standard deviations from mean. Data are representative of at least two independent experiments done in duplicate.