Figure 6. WhiB4 regulates response to AG in Mtb.
(A) Cumulative node weight intensities (CNW) of different functional classes regulated by WhiB4 upon AG treatment. (B–G) Heat maps depicting expression of genes (log2fold-change, p≤0.05) coordinating cell wall processes, alternate respiration and CCM, antioxidants, DNA repair, PE and PE_PGRS and drug efflux pumps in case of Mtb and MtbΔwhiB4 treated with AG for 6 hr as described in Materials and methods. Cumulative node weight intensities for different functional classes are available in Figure 6—source data 1.
DOI: http://dx.doi.org/10.7554/eLife.25624.017
Figure 6—source data 1. Cumulative node weight intensities for different functional classes as depicted in Figure 6A.
elife-25624-fig6-data1.xlsx (9.7KB, xlsx)
DOI: 10.7554/eLife.25624.018
Figure 6—figure supplement 1. qRT-PCR analysis of whiB4 expression in MtbΔmshA, mshA-comp, and mshA-OE strains.
Fold change was measured with respect to untreated wt Mtb by normalizing expression with the 16srRNA transcript. Error bars represent standard deviations from mean. Data are representative of at least two independent experiments done in duplicate.
Figure 6—figure supplement 2. qRT-PCR analysis of MtbΔwhiB4 exposed to 10X AG for 6 hr.
Fold change for each transcript was measured with respect to wt Mtb exposed to 10X AG for 6 hr by normalizing expression with the 16srRNA transcript. Error bars represent standard deviations from mean. Data are representative of at least two independent experiments done in duplicate.
Figure 6—figure supplement 3. Vancomycin-BODIPY staining of different Mtb strains.
(A) Wt Mtb, MtbΔwhiB4, and whiB4-OE were grown to OD600 nm of 0.6 and incubated with 1 µg/ml of Vancomycin-BODIPY for 16 hr and visualized by confocal microscopy (63X). The scale of images is 3 µm (B) Scatter plot showing the cell measurement for above mentioned strains. Each dot represents one cell (n > 150).