Figure 7. WhiB4 regulates AG-induced oxidative shift in EMSH of Mtb both in vitro and during infection.
(A) MtbΔwhiB4 and whiB4-OE expressing Mrx1-roGFP2 were treated with lethal (10X MIC) and sub-lethal (0.2 X MIC) concentrations of AG and ratiometric response was measured by flow cytometry at indicated time points. (B) PMA differentiated THP-1 cells were infected with MtbΔwhiB4 and whiB4-OE expressing Mrx1-roGFP2 (MOI:10) and treated with indicated concentrations of Amox in the presence of Clav (8 µg/ml) as described in Materials and methods. At indicated time points, ~30,000 infected macrophages were analyzed by flow cytometry to quantify changes in Mtb subpopulations displaying variable EMSH as described in Materials and methods. *p≤0.05, **p≤0.01 and ***p≤0.001.