Figure 4.

Functional Assessment of Mutant β-Catenin on Transcription using the TOPflash Luciferase Activity Assay

STF cells were transiently transfected with expression constructs for wild-type (WT) or mutant β-catenin (p.Arg710Cys or p.His720^∗) or empty vector (pDEST40) alongside a Renilla luciferase plasmid and luciferase activity was measured 48 hr later. The bars represent the firefly/Renilla luciferase ratios for the different constructs. The results are from three independent experiments performed in triplicate. Error bars depict SEM; ^∗∗p < 0.01, ^∗∗∗p < 0.0001.