Table 1. Results of molecular genetic tests for the role of swnK in swainsonine biosynthesis.
Strain | Genotype | Comment | Swainsonine Concentration (µg/g Dry Mass ± SD) |
---|---|---|---|
ARSEF 2575 | WT | Wild-type strain | 653 ± 119 |
∆swnK | ∆swnK | swnK disruption mutant | Not detected |
G11 | WT + bar | Ectopic transformant | 620 ± 103 |
G24 | WT + bar | Ectopic transformant | 633 ± 81 |
C5 | ∆swnK + swnK | Complemented mutant | 1268 ± 470 |
C12 | ∆swnK + swnK | Complemented mutant | 1141 ± 497 |
C18 | ∆swnK + swnK | Complemented mutant | 145 ± 46 |
C19 | ∆swnK + swnK | Complemented mutant | 2573 ± 1408 |