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. 2017 Apr 28;7(6):1799–1809. doi: 10.1534/g3.117.041053

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Copyright © 2017 Dacquay et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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NuA4 mutants exacerbate the growth defects of sec14-1^ts on inositol-depleted media. NuA4 mutants (A) eaf1Δ, (B) eaf7Δ, and (C) esa1-L254P^ts increase the growth defects of the SEC14 temperature-sensitive mutant, sec14-1^ts, under inositol-depleted conditions. Wild-type (YKB1079), sec14-1^ts (YKB3144), eaf1Δ (YKB3333), sec14-1^tseaf1Δ (YKB3935), eaf7∆ (YKB3292), sec14-1^tseaf7∆ (YKB4068), esa1-L254P^ts (YKB4236), and sec14-1^tsesa1-L254P^ts (YKB4242) cultures were grown to midlog phase in SC-inositol (+75 µM myo-inositol) prior to being diluted to an OD₆₀₀ of 0.1, and four times 10-fold serial dilutions were spotted on inositol-supplemented (75 µM myo-inositol) SC media or inositol-depleted SC media. Plates were incubated for 2 d at 30, 32, or 33.5° and images are representative of three biological replicates.