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. 2017 Apr 19;7(6):1819–1832. doi: 10.1534/g3.117.040147

Figure 3.

Figure 3

Functional validation of the role of CYP9J11 P450 gene in carbamate/pyrethroid resistance. (A) Percentage depletion of 20 µM carbamate and pyrethroid insecticides with CYP9J11. Results are an average of three replicates (n = 3) compared with negative control. * and ** Significantly different from negative control (−NADPH) at P < 0.05 and P < 0.01, respectively. (B) Polar metabolites with short retention time eluted at the beginning of chromatogram of CYP9J11 metabolism of bendiocarb (NADPH+). A third putative metabolite of bendiocarb metabolism eluted at 18.045 min. (C) Chromatogram of NADPH- incubation tubes devoid of polar metabolites with short retention indicating no metabolism of bendiocarb in the absence of NADPH regeneration agent. (D, E) Michaelis-Menten plot of CYP9J11 mediated metabolism of permethrin and deltamethrin, respectively. Results are an average of three replicates (n = 3) compared with negative control; (F) Allosteric sigmoidal curve of CYP9J11 metabolism of bendiocarb. Results are average of three replicates (n = 3) compared with negative control. Khalf = KM; h = 2.29.