Figure 2.

Growth rates on glycine as sole nitrogen source for selected members of the SLC25 family in combination with HEM25 deficiency. Cells of the indicated genotypes were grown overnight on SD-Ura medium containing 1 g/liter of ammonium sulfate at 30°, washed twice and inoculated at OD_{600 nm} 0.1 in SD-Ura medium containing 30 g/liter of glycine. Cells were cultivated at 30°, and growth was monitored spectrophotometrically. Differences between single deletion strains and double deletion strains were determined using ANOVA test with randomized factors. At least three independent experiments were done to calculate the p values, mean, and SEM. The numbers represented in the graph are growth rates calculated over a period of 4 d.