Figure 3.

Heme content for selected members of the SLC25 family in combination with HEM25 deficiency. Cells of the indicated genotypes were grown at OD_{600 nm} 0.6–1 in SD-Ura. Cells were harvested and processed for heme determination. Differences between single deletion strains and double deletion strains were determined using ANOVA test with randomized factors. At least three independent experiments were done to calculate the p values, mean, and SEM. Values are normalized to wild type (WT). WT heme level was 28.9 ± 4.2 fmol/μg of protein.