Figure 4.

Glycine and 5-Ala alleviate the growth defect of a subset of SLC25 family members upon inactivation of the mitochondrial glycine importer HEM25. Yeast strains of the indicated genotypes were grown to early stationary phase in SC-Ura medium supplemented with 5 mM glycine and 0.38 mM 5-Ala to keep the double mutant cells growing without impediment. Cells were washed and resuspended in sterilized water to OD_{600 nm} 0.4, then serially diluted (1:10) and spotted on SD-Ura solid media containing dextrose as carbon source. Plates were imaged after 3 d incubation at 30°.