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. 2017 Apr 12;7(6):1861–1873. doi: 10.1534/g3.117.041194

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Copyright © 2017 Dufay et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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High molecular weight aggregates are present in mitochondria from flx1Δ hem25Δ cells. Cells of the indicated genotypes were grown to an OD of 1.0 in defined medium with raffinose. Cells were then transferred and grown in lactate for 5 hr. Cells were harvested, mitochondria were isolated and mitochondrial protein complexes were solubilized with digitonin. Protein complexes were resolved by BN-PAGE, transferred into PVDF and analyzed by western blotting using antibodies specific for Cox2 (upper and middle panels; they are identical, middle panel was a longer exposure) or F1α (lower panel). Asterisk denotes the position of high molecular weight aggregates.