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. 2017 Jun 16;7:3756. doi: 10.1038/s41598-017-03998-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Metabolite analysis. (a) Radio-HPLC chromatogram of the [⁶⁴Cu]Cu-NOTA-HsTX1[R14A], (b) Radioluminogram of ⁶⁴Cu in a TLC analysis of rat arterial blood plasma samples of 1, 3, 5, 10, 20, 30, 120 min and 4 h, urine and kidney extract samples at 4 h p.i.; (c) Radioactive metabolite fraction in the arterial blood plasma samples, kidney extract and urine expressed as % of original compound.