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. 2017 Jun 16;7:3697. doi: 10.1038/s41598-017-03928-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Time-course of adenoviral transduction in the basal hypothalamus. (A–L) Frontal sections (40 µm) from the hypothalamus at 18 h (A–C), 24 h (D–F) and 48 h (G–I) following injection of adenoviral particles (Ad-shGK-EGFP) into the 3V. EGFP fluorescence (green) and immunoreactivity for vimentin (red) and colocalization of EGFP and vimentin (yellow) are shown. Arrows show the processes of transduced tanycytes. (G) Panoramic view of the basal hypothalamus, showing tanycyte transduction. (I) Higher magnification image showing that Ad-EGFP transduction occurs in β2-tanycytes. AN, arcuate nucleus; 3V, third ventricle; ME, median eminence. Scale bars (A–F and H) 50 µm, (G) 100 µm, (I) 20 µm.