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. 2017 Jun 16;7:3697. doi: 10.1038/s41598-017-03928-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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GK inhibition 48 h after AdshGK injection into the 3V. (A) Western blot assays of hypothalamic protein extracts in control (Ad-shβGal-injected rats) and Ad-shGK-injected rats using anti-GK, anti-GLUT2, anti-GKRP, and anti-GFP (transduction control) antibodies. An anti-β-actin antibody was used as loading control. Images are representative of three different experiments. (B) Densitometric analysis of each protein relative to β-actin evaluated in Ad-shGK-injected rats, as a percentage of this protein in Ad-shβgal-EGFP-injected rats. Statistics: t-test with Welch correction *p < 0.05.