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. 2017 Jun 16;7:3744. doi: 10.1038/s41598-017-03763-0

Figure 1.

Figure 1

TCRP1 is upregulated by c-Myc in tongue and lung cancer cells. (A) A sketch map of potential TCRP1 promoter predicted by several online program. The transcriptional start site (TSS) of TCRP1 promoter was defined as‘+1’. (B) A schematic representation of c-Myc binding site in putative TCRP1 promoter. C-Myc also is known as Myc. (C) ChIP assay was performed in tongue cancer cell Tca8113/PYM. Pol IIcontacts to polymerase II. (D) TCRP1 and c-Myc expression were measured by real-time RT-PCR and western blot assay in tongue and lung cancer cells. **P < 0.01. (E,F) Plasmid pMxs-h-c-Myc and control pMxs-h were transfected into tongue cancer cell Tca8113 and lung cancer cell A549, siRNAs targeting c-Myc together with its control were transfected into Tca8113/PYM and A549/DDP cells, 48 h later, the mRNA and protein expression levels of TCRP1 and c-Myc were estimated in these cells. **P < 0.01. (G) A schematic diagram of c-Myc wild type and mutant binding site in TCRP1 promoter. Dual-luciferase reporter gene assay was conducted in HEK-293T, tongue and lung cancer cells with overexpression c-Myc or siRNA targeting c-Myc. **P < 0.01.