Table 1.
Structural parameters obtained by model fitting.
| d54-DMPC:DMPG | Inner Headgroup (Å) | Inner Chain (Å) | Outer Chain (Å) | Outer Headgroup (Å) | Total bilayer thickness (Å) | Nw | d54-DMPC ratio in the inner leaflet | Scale factor | background | Polydispersity |
|---|---|---|---|---|---|---|---|---|---|---|
| 0.75:0.25 Lipid only | 8.2 | 14.3 | 14.5 | 9.3 | 46.3 ± 1.0 | 8.0 | 81.6 ± 0.2% | 0.012 | 0.0096 | 0.369 |
| 0.75:0.25 P/L = 1/100 | 8.3 | 14.5 | 14.0 | 9.0 | 45.8 ± 1.0 | 9.0 | 82.5 ± 0.2% | 0.014 | 0.0104 | 0.384 |
| 0.75:0.25 P/L = 1/30 | 7.5 | 14.6 | 14.1 | 9.1 | 45.3 ± 1.0 | 6.4 | 85.0 ± 0.2% | 0.012 | 0.0100 | 0.348 |
| 0.75:0.25 P/L = 1/10 | 6.8 | 14.0 | 14.0 | 9.5 | 44.3 ± 1.0 | 7.5 | 87.0 ± 0.3% | 0.014 | 0.0119 | 0.329 |
| 0.5:0.5 Lipid only | 8.0 | 13.5 | 11.7 | 9.3 | 42.5 ± 1.0 | 4.8 | 63.4 ± 0.2% | 0.012 | 0.0136 | 0.300 |
| 0.5:0.5 P/L = 1/100 | 6.0 | 13.7 | 11.4 | 9.5 | 40.6 ± 1.0 | 1.5 | 66.3 ± 0.2% | 0.011 | 0.0108 | 0.393 |
| 0.5:0.5 P/L = 1/30 | 6.3 | 13.5 | 12.7 | 9.3 | 41.8 ± 1.0 | 3.0 | 70.7 ± 0.2% | 0.012 | 0.0116 | 0.300 |
| 0.5:0.5 P/L = 1/10 | 6.0 | 14.5 | 14.5 | 9.5 | 44.5 ± 2.5 | 5.0 | 71.8 ± 0.3% | 0.014 | 0.0136 | 0.300 |
The total bilayer thickness is the sum of the head group and the chain thickness of both inner and outer leaflets. The thickness of various shells is correlated; therefore, the total bilayer thickness and its uncertainty are more meaningful parameters. Nw is the number of water associated per lipid head group.