Table 6.

Sequences, size and GC contents of primers used for PCR amplification.

Primers	Sequence (5′-3′)	Nucleotide numbers in E. coli 16S rDNA sequence	Primer size (bp)	GC content (%)
EfacF1	CGTTAGTAACTGAACGTC	470–488	18	44.44
Efac R1	GACCGCGAGGTCATGCA	1353–1370	17	64.7
Universal 1492R	GGATACCTTGTTACGACTT	1473–1492	19	42.1