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. 2017 Jun 16;7:3693. doi: 10.1038/s41598-017-03540-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Natural variation in A. thaliana Zn and Cd tolerance. 23 accessions of the Versailles Core24 were grown on vertical agar plates under control conditions or in the presence of either 5 µM CdCl₂ (a) or 50 µM ZnSO₄ (b). Length of primary roots was determined after 12–14 d. Relative root growth of seedlings as an indicator for tolerance was calculated as follows: root length metal treatment/mean root length control conditions × 100. Absolute root lengths (in mm) under control conditions were: Bl-1: 64 ± 17; Stw-0:66 ± 26; N13: 69 ± 26; Akita: 70 ± 18; Shak: 77 ± 19; St-0: 82 ± 14; Blh-1: 83 ± 16; Ct-1: 84 ± 19; Kn-0: 85 ± 20; Can-0: 85 ± 16; Mh-1: 87 ± 18; Col-0: 88 ± 15; Ply-1: 90 ± 22; Cvi-0: 90 ± 14; Oy-0: 91 ± 23; Edi-0: 91 ± 16; JEA: 92 ± 22; Tsu-0: 94 ± 21; Ler-0: 94 ± 24; Mt-0: 96 ± 17; Bur-0: 99 ± 22; Gre-0: 102 ± 23; Alc-0: 103 ± 20). Data represent means ± SD. Per accession 17 to 185 plants were compared. The average n was 119. Statistical analysis was performed via one-way ANOVA and data were grouped based on Tukey’s 95% confidence intervals.