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. 2017 Jun 16;7:3645. doi: 10.1038/s41598-017-03900-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The CoMBI system. (a) Photograph of the system. The camera (c) is placed in front of the cryostat. The controller (d) is attached to the front panel of the cryostat. The sensor (e) is attached close to the cryostat handle to detect the handle position. The illumination (f) is set in the chamber. (b) An example of the block-face image shows the clear outer circumference. (c–g) Circuit of the system: camera (c), controller (d), sensor (e), illumination (f), cleaning brush (g). An ATtiny85 microcontroller (left) receives signals about the position of the cryostat handle from the sensor (e), and triggers release of the camera shutter (c) using an optocoupler. The illumination (f) consists of two LED lamps on the door to illuminate the block-face orthogonally. The cleaning brush (g) consists of a servomotor and three brushes attached to the back of the knife holder to clean the block-face during imaging. Bar in b: 5 mm.