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. 2017 Jun 16;7:3645. doi: 10.1038/s41598-017-03900-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Correlation between 2D images and MPR images of E10.5 mouse embryo. A mouse embryo was imaged by the CoMBI system and shown as MPR images. (a–c) Block-face images. (a–c) Frozen sections were collected at the positions of (a–c). (g,h) The sagittal planes were made by MPR from 565 block-face images. The nervous, cardiovascular, and urogenital systems are shown. (i) Reconstructed coronal planes of the thorax show the respiratory system and vascular system. (j) Reconstructed plane of the abdomen shows gastrointestinal tract. (k) Reconstructed plane of the head shows sensory organs. 1: myelencephalon, 2: otic vesicle, 3: trigeminal ganglion, 4: diencephalon, 5: neural tube, 6: spinal ganglion, 7: somite, 8: trachea, 9: dorsal aorta, 10: anterior cardinal vein, 11: atrium, 12: ventricle, 13: foramen ovale, 14: olfactory pit, 15: telencephalon, 16: urogenital ridge, 17: mesonephron, 18: forelimb bud, 19: hindlimb bud, 20: mesencephalon, 21: mandible, 22: liver, 23: superior mesenteric artery, 24: umbilical artery, 25: intestine, 26: 3rd aortic arch, 27: 4th aortic arch, 28: 6th aortic arch, 29: bronchus, 30: lens, 31: retina, 32: optic stalk, 33: Rathke’s pocket. Bars: 1 mm. Note that labels 5, 6, 9, 11–14, and 17 indicate structures on lines b and c for correlation between H & E stained sections (e,f) and MPR images (g,h).