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. 2017 Jun 16;7:3673. doi: 10.1038/s41598-017-03825-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Mutant CCT5-H147R shows the same double homo-octameric ring arrangement as CCT5-WT. The CCT5-H147R structure demonstrated the Arg147 residue does not point into the inter-rings or intra-ring (between subunits) interfaces. (b) A 2mF_o-DF_c electron density map for CCT5-H147R around the side chain of Arg147 and Ser428. The broken line represent the hydrogen bond distance between NE atom of Arg147 located at helix-5 and the main carbonyl group of Ser428 located at helix-13 of equatorial domain.