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. 2017 Jun 16;7:3695. doi: 10.1038/s41598-017-03052-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PTEN protein phosphatase activity is required for inhibition of HCV replication in HCV genomic replicon cells. (a and b) Huh-7 cells with an HCV-2a J6/JFH-1(p7-rLuc-2A) genomic replicon were transfected with plasmids expressing wild-type Flag-PTEN (PTEN WT), truncated mutants, phosphatase deficient mutants, or empty vector. Forty eight hours after transfection, luciferase activities (a) or HCV RNA levels (b) were determined. (c) The protein levels of PTEN and β-actin were determined by Western blotting using anti-PTEN and anti-β-actin antibodies, respectively. (d and e) Huh-7 cells with an HCV-2a JFH-1 rLuc subgenomic replicon were transfected with plasmid vector or PTEN expressing plasmid. Luciferase (d) and HCV RNA levels (e) were determined 48 hours after transfection. Data were expressed as fold changes relative to vector control; *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, or NS for not significant.