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. 2017 Jun 16;7:3695. doi: 10.1038/s41598-017-03052-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PTEN interacts with the domain I of HCV core. (a) Huh-7 cells harboring HCV-2a J6/JFH-1(p7-rLuc-2A) replicon were transfected with plasmids expressing GST, GST-PTEN wild-type or GST-PTEN mutants. At 48 hours after transfection, cell lysates were subjected to GST pull-down assay. Input and pull-down products were analyzed by Western blotting (WB) using anti-HCV core and anti-GST antibodies, respectively. (b) Purified His₆-BFP (blue fluorescent protein), His₆-HCV core, GST and GST-PTEN proteins were subjected to GST pull-down assay. Pull-down products were analyzed by Western blotting (WB) using anti-His₆, anti-GST and anti-PTEN antibodies, respectively. Western blot image with a “shorter exposure” can be found in the Supplementary Information. (c and d) Huh-7 cells were co-transfected with plasmids expressing Flag-tagged HCV core, either full-length, domain I (D-I; aa. 1–118), or domain II (D-II, aa. 119–178), together with plasmids expressing GST (c) or GST-PTEN (d). The core proteins were expressed as fusion proteins with EGFP. Flag-tagged EGFP-expressing plasmid was included as negative control. At 48 hours after transfection, cell lysates were subjected to GST pull-down assay. Input and pull-down products were analyzed by Western blotting (WB) using anti-Flag and anti-GST antibodies, respectively.