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. 2017 Jun 16;7:3650. doi: 10.1038/s41598-017-03983-4

Figure 4.

Figure 4

EM images FtsZ and FtsZ-ZipA assembled with EDTA and GTP, pH 6.5 (A–C), or in HMK pH 7.5 with GMPCPP (D,E). In MEK buffer (50 mM MES, pH 6.5, 100 mM KAc, 1 mM EDTA) plus GTP, a few filaments were assembled with 5 µM FtsZ (A) and more with 10 µM FtsZ (B). 5 µM FtsZ plus 5 µM ZipA in MEK assembled abundant thick filament bundles (C). In HMK buffer, pH 7.5, in 0.5 mM GMPCPP, 5 µM FtsZ assembled into long filaments and bundles (D). 5 µM FtsZ plus 5 µM ZipA in GMPCPP, pH 7.5, assembled into long filaments with a tendency to curve (E). Bar represents 100 nm.