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Assembly of FtsZ plus FtsA or FtsA*, at pH 7.5. (A,B) Assembly in 0.5 mM GDP of 15 µM FtsZ plus 10 µM FtsA (A) or 10 µM FtsA* (B). FtsA* caused assembly of abundant minirings. (C–E) Assembly in 1 mM GTP of 10 µM FtsZ plus 10 µM FtsA* without ATP (C,D), and with 1 mM ATP (E). Bars are 100 nm in A–D and 500 nm in E.
