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. 2017 Jun 16;7:3650. doi: 10.1038/s41598-017-03983-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Dynamics of the FtsZ assembly and filament turn-over in MMK buffer, pH 6.5. (A) FtsZ assembly kinetics measured by Atto fluorescence. Assembly is almost the same for FtsZ alone and with ZipA and FtsA*. (B) FtsZ assembly kinetics measured by light-scattering. The enhanced light scattering with ZipA and FtsA* reflects bundling. (C) Direct comparison of the assembly kinetics of FtsZ-ZipA mixture measured by Atto fluorescence and light-scattering. Filament assembly reported by ATTO fluorescence precedes the bundling reported by light scattering. (D) The kinetics of FtsZ filament turn-over measured by FRET technique. The turn-over rates are slower for polymers with ZipA or FtsA*.