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. 2017 Jun 16;7:3652. doi: 10.1038/s41598-017-03899-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Osteogenesis detection in bone defect areas. (a) The expression of osteogenesis related genes on the 7th postoperative day, including OCN, BMP-2 and RUNX2. These three genes were significantly increased in group A compared with the other two groups. *P < 0.05; (b) Haematoxylin and eosin staining of bone defect areas on the 7th postoperative day. The white arrow indicated the bone defect edge. More new trabecular bone (green arrow) in group A; (c) Three-dimensional micro-CT images of bone defect areas. The white line dotted the region of interest area. More new bone (green arrow) were found in group A.