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. 2017 Jun 16;7:3709. doi: 10.1038/s41598-017-04011-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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RUFY3 synergizes with FOXK1 to promote tumor metastasis in vivo. (A) External whole-body fluorescence images of the liver by injection of LoVo/pEGFP-N1(Vector), LoVo/pEGFP-RUFY3-scr-shRNA and LoVo/pEGFP-RUFY3- FOXK1-shRNA were obtained 30 days after spleen injection. The mice were sacrificed. (B) Metastatic cancer tissues were stained with H&E. (C) Vimentin expression in the CRC-derived liver metastases was detected by IHC. (D) Vimentin expression in tumors derived from LoVo cells was determined by qRT-PCR; ***P < 0.001. Scale bars, 100 μm in (B,C).