Table 1.
PCR conditions and primer sequence for examined genes
| mRNA expression | Forward primer (5′-3′) | Reverse primer (5′-3′) | PCR cycles and Annealing |
|---|---|---|---|
| PEPCK (236 bp) | TTTACTGGGAAGGCATCGAT | TCGTAGACAAGGGGGCAC | 30 cycles, 52 °C 1 min |
| PK (229 bp) | ATTGCTGTGACTGGATCTGC | CCCGCATGATGTTGGTATAG | 30 cycles, 52 °C 1 min |
| ACO (633 bp) | GCCCTCAGCTATGGTATTAC | AGGAACTGCTCTCACAATGC | 35 cycles, 52 °C 1 min |
| CPT-1 (628 bp) | TATGTGAGGATGCTGCTTCC | CTCGGAGAGCTAAGCTTGTC | 35 cycles, 52 °C 1 min |
| PPAR γ (550 bp) | CATTTCTGCTCCACACTATGAA | CGGGAAGGACTTTATGTATGAG | 33 cycles 52 °C 1 min |
| PPAR-α (680 bp) | GAGGTCCGATTCTTCCACTG | ATCCCTGCTCTCCTGTATGG | 35 cycles, 58 °C 1 min |
| FAS (345 bp) | CCAGAGCCCAGACAGAGAAG | GACGCCAGTGTTCGTTCC | 37 cycles, 61 °C 45 s |
| SREBP-1c (191 bp) | GGAGCCATGGATTGCACATT | AGGAAGGCTTCCAGAGAGGA | 35 cycles, 58 °C 50 s |
| HMG-CoAR (467 bp) | CCTGCTGCCATAAACTGGAT | GCCATTACAGTGCCACACAC | 31 cyc les58°C 1 min |
| GST (575 bp) | GCTGGAGTGGAGTTTGAAGAA | GTCCTGACCACGTCAACATAG | 35 cycles, 55 °C 1 min |
| CYP7A1 (574 bp) | CCTCCTGGCCTTCCTAAATC | GTACCGGCAGGTCATTCAGT | 30 cycles 58 °C 1 min |
| SOD (410 bp) | AGGATTAACTGAAGGCGAGCAT | TCTACAGTTAGCAGGCCAGCAG | 33 cycles, 55 °C 1 min |
| GAPDH (309 bp) | AGATCCACAACGGATACATT | TCCCTCAAGATTGTCAGCAA | 25 cycles, 52 °C 1 min |