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Comparison of alignment criteria of Ub-ISAP and VISA
| Alignment criteria | Ub-ISAP | VISA |
|---|---|---|
| Alignment score | 100 | >60 |
| Percent identity | 100 | >92 |
| IS distance from query start site (QSS) | 0 bp | 3 bp |
| Acceptance for IS calling | Reads aligning at more than one position are rejected | Top 5 candidates highest alignment score) |
