Table 2. Characteristics of 17 polymorphic EST-SSR markers.
| Locus | Primer sequence 5’–3’ | Repeat motif | NA | Size range (bp) | HE | HO | HWEa | PIC | GenBank accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BC1 | F:GCAGTTCTGTGCAGTACCATACAT | (TA)6 | 4 | 172–182 | 0.065 | 0.038 | 0.036* | 0.193 | Pr032805680 |
| R:GGCTTCTGATCAGGTTTCTGAAT | |||||||||
| BC2 | F:GAGATCCCAGATCCAGATCTTCT | (TC)6 | 3 | 160–164 | 0.038 | 0.023 | 0.192 n.s | 0.423 | Pr032805689 |
| R:AACATTAATGGAAACACGTCGTC | |||||||||
| BC3 | F:ATTCGCTCTCTTGGTATGACTGT | (TA)6 | 5 | 170–184 | 0.054 | 0.045 | 0.380n.s | 0.664 | Pr032805690 |
| R:CCCAATTTGAAGTGTTGCTTTAC | |||||||||
| BC4 | F:TATCAGCGTGTGTGAATAGTTGC | (TA)7 | 4 | 157–163 | 0.097 | 0.045 | 0.004** | 0.491 | Pr032805691 |
| R:TAACCTAAATTCGAATCCATCCA | |||||||||
| BC5 | F:CAAACTTGGCTTAATACCATTCG | (TG)9 | 3 | 119–125 | 0.079 | 0.083 | 0.713 n.s | 0.469 | Pr032805692 |
| R:CCATGATCATCTCTATTTCAGGC | |||||||||
| BC6 | F:CCTTAAGGAGATGCATTGTGAAT | (TC)9 | 3 | 159–169 | 0.000 | 0.000 | - n.c. | 0.299 | Pr032805693 |
| R:GTATGAAGGGCATCAACAATAGG | |||||||||
| BC7 | F:GCTGAAAGTTGGTGATTGCTAGT | (AT)9 | 4 | 166–178 | 0.120 | 0.125 | 0.087 n.s | 0.526 | Pr032805694 |
| R:AGTTATGTCTTCGCTTGCTTCAG | |||||||||
| BC8 | F:AACGTGAGAGTGCTAGTTCGGTA | (TGA)5 | 3 | 167–173 | 0.014 | 0.000 | 0.041* | 0.17 | Pr032805695 |
| R:TCTTCCTCACTTTATCATCCACG | |||||||||
| BC9 | F:AGAAGAGGTACGACAGTTTGCTG | (GCG)5 | 2 | 156–159 | 0.059 | 0.064 | 1.000 n.s | 0.195 | Pr032805696 |
| R:TTCACGTCCGAATTCTTAGTCTC | |||||||||
| BC10 | F:CACTGCACATAGAAGGAGGAGTT | (GCG)6 | 5 | 108–129 | 0.081 | 0.076 | 0.146 n.s | 0.581 | Pr032805681 |
| R:GTAATCGCCTACATGATTCATCC | |||||||||
| BC11 | F:CAGCAGTATGTCGGGATTATTTC | (TTTCT)4 | 2 | 123–133 | 0.000 | 0.000 | -n.c. | 0.155 | Pr032805682 |
| R:CCTCTGGTCATATTGCTGTTACC | |||||||||
| BC12 | F:AACAAGAGGGTCAGCTACAACAG | (CAGCAA)4 | 4 | 160–178 | 0.104 | 0.095 | 0.184 n.s | 0.549 | Pr032805683 |
| R:CAGCAATGGTATTAGCAGAGGAC | |||||||||
| BC13 | F:ACCTTGACGATCCTTCATCTTCT | (GGTGCG)4 | 6 | 132–174 | 0.124 | 0.095 | 0.161 n.s | 0.701 | Pr032805684 |
| R:TTATGTTCTCCATATCCGTCAGC | |||||||||
| BC14 | F:GGCAGCAATATAGCTCAAATACG | (GACAAG)4 | 4 | 170–188 | 0.196 | 0.083 | 0.000*** | 0.516 | Pr032805685 |
| R:ACCTGATCGTTCACAACTTCATC | |||||||||
| BC15 | F:TCTTATTCAACACAACAGCCTGA | (ATGATA)4 | 5 | 151–175 | 0.157 | 0.140 | 0.228 n.s | 0.528 | Pr032805686 |
| R:GCTGCAGTTGATAATGAGAAGGA | |||||||||
| BC16 | F:ACCAATGGTCTATATTTCAACGG | (ATTACT)4 | 6 | 149–179 | 0.132 | 0.125 | 0.174 n.s | 0.643 | Pr032805687 |
| R:GTGCCCCACATAGCTTCTATCTA | |||||||||
| BC17 | F:TGACGAGGCTTCTACAGAATGAG | (CATCCT)4 | 2 | 137–143 | 0.034 | 0.045 | 1.000 n.s | 0.186 | Pr032805688 |
| R:ACAAACAACAAGATGGGAATCAT |
Notes.
- NA
- number of alleles per locus across all populations
- HE
- expected heterozygosity (mean value)
- HO
- observed heterozygosity (mean value)
- PIC
- polymorphic information content
- HWE
- Hardy-Weinberg equilibrium
a
After Bonferroni correction, significant departures from Hardy–Weinberg equilibrium are indicated by ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001. n.s., not significant; n.c., not calculated (Clarke & Gorley, 2001).