a Hybrid F98:F98CD spheroids with constant number of F98 cells (4×103) and increasing number of prodrug converting F98CD cells as shown on the figure, incubated with 5-FC (0.2 μg/ml) with and without PDT. PDT was performed after 5-FC was administered. b PDT was performed before 5_FC was administered.
Light treatment in both cases was performed with 0.8J/cm2, λ= 670nm. Each data point represents spheroid volume after 2 weeks in culture as a % of controls at day 14. Mean of 3 experiments, error bars denote standard errors. Asterisks (*) denote significant differences (p<0.05) compared to control value.