Table 3.
Features of the gene polymorphisms used in the study.
| Single nucleotide polymorphism | Nucleotide substitution | Chromosomal position | Amino acid substitution | Forward 5′-3′ and reverse 3′-5′ polymerase chain reaction primers |
|---|---|---|---|---|
| IL1B gene | ||||
| rs1143634 | G > A | 113590390 | Phe105Phe | F: cataagcctcgttatcccatgtgtc R: aagaagataggttctgaaatgtgga |
| IL6 gene | ||||
| rs1554606 | T > G | 22768707 | Intronic | F: ttagttcatcctgggaaaggtactc R: cagggccttttccctctctggctgc |
| rs1800796 | G > C | 22766246 | 5′-upstream | F: atggccaggcagttctacaacagcc R: ctcacagggagagccagaacacaga |
| rs2069827 | G > T | 22765456 | 5′-upstream | F: gcccaacagaggtcactgttttatc R: atcttgaagagatctcttcttagca |
| IL6R gene | ||||
| rs2228145 | A > T/C | 154426970 | Asp358Val/Ala | F: aattttttttttaacctagtgcaag R: ttcttcttcagtaccactgcccaca |
| rs2229238 | T > C | 154437896 | 3′-UTR | F: ccagcagcctggaccctgtggatga R: aaaacacaaacgggctcagcaaaag |
| IL8 gene | ||||
| rs2227306 | C > T | 74607055 | Intronic | F: aactctaactctttatataggaagt R: gttcaatgttgtcagttatgactgt |
| IL10 gene | ||||
| rs1800871 | A > G | 206946634 | 5′-upstream | F: agtgagcaaactgaggcacagagat R: ttacatcacctgtacaagggtacac |
| rs1800872 | T > G | 206946407 | 5′-upstream | F: ttttactttccagagactggcttcctacag R: acaggcggggtcacaggatgtgttccaggc |
| rs1800896 | T > C | 206946897 | 5′-upstream | F: tcctcttacctatccctacttcccc R: tcccaaagaagccttagtagtgttg |
| IL12B gene | ||||
| rs3212227 | T > G | 158742950 | 3′-UTR | F: attgtttcaatgagcatttagcatc R: aactatacaaatacagcaaagatat |
| IL12RB gene | ||||
| rs375947 | A > G | 18180451 | Met365Thr | F: aggctgccattcaatgcaatacgtc R: tgctctgagcccgggctggccaata |
| TNF gene | ||||
| rs361525 | G > A | 31543101 | 5′-upstream | F: ggcccagaagacccccctcggaatc R: gagcagggaggatggggagtgtgag |
| rs1800629 | G > A | 31543031 | 5′-upstream | F: gaggcaataggttttgaggggcatg R: ggacggggttcagcctccagggtcc |
| CRP gene | ||||
| rs3093077 | A > C | 159679636 | Not announced | F: ggaatccaggcaagtacgacaaccc R: tctgagactagtgggcagttgtcct |
| rs1130864 | G > A | 159683091 | 3′-UTR | F: cctcaaattctgattcttttggacc R: tttcccagcatagttaacgagctcc |
| rs1205 | C > T | 159682233 | 3′-UTR | F: acttccagtttggcttctgtcctca R: agtctctctccatgtggcaaacaag |
| APOB gene | ||||
| rs1042031 | C > T | 21225753 | Glu4181Lys | F: caatcagatgcttgactttcatatggaatt R: ttgagtaactcgtaccaagccatcaaacac |
| rs6725189 | G > T | 21219001 | Not announced | F: ttcccagcctcagctcaacagagctatggg R: cagcagtcggccctctctattgttctttcc |
| APOE gene | ||||
| rs7412 | C > T | 45412079 | Arg176Cys | F: ctcctccgcgatgccgatgacctgcagaag R: gcctggcagtgtaccaggccggggcccgcg |
| rs429358 | T > C | 45411941 | Cys130Arg | F: gcccggctgggcgcggacatggaggacgtg R: gcggccgcctggtgcagtaccgcggcgagg |
| LIPC gene | ||||
| rs1800588 | C > T | 58723675 | 5′-upstream | F: tctttgcttcttcgtcagctccttttgaca R: gggggtgaagggttttctgcaccacacttt |
| LPA gene | ||||
| rs10455872 | A > G | 161010118 | Intronic | F: tcagacaccttgttctcagaaccca R: tgtgtttatacaggttagaggagaa |
| NOTCH1 gene | ||||
| rs13290979 | A > G | 139425634 | Intronic | F: ccagcccagcagtgaagaaactgagcccac R: accctcctggcctgacctacactcgggctt |
| VDR gene | ||||
| rs2228570 | A > G | 48272895 | Met1Thr/Lys/Arg | F: ggcagggaagtgctggccgccattgcctcc R: tccctgtaagaacagcaagcaggccacggt |
| CASR gene | ||||
| rs1042636 | A > G | 122003769 | Arg990Gly | F: gatgagcctcagaagaacgccatggcccac R: ggaattctacgcaccagaactccctggagg |
| OPG gene | ||||
| rs3134069 | A > C | 119964988 | 5′-upstream | F: ggagcttcctacgcgctgaacttctggagt R: gcctcctcgaggtctttccactagcctcaa |
| rs2073618 | G > C | 119964052 | Asn3Lys | F: gggacttaccacgagcgcgcagcacagcaa R: ttgttcattgtggtccccggaaacctcagg |
| rs3102735 | T > C | 119965070 | 5′-upstream | F: ctttgctctagggttcgctgtctcccccat R: aattccctggtctagaagttagacttgatg |
| CALCR gene | ||||
| rs1801197 | A > G | 93055753 | Leu481Pro | F: tcgccttggttgttggctggttcattcctc R: gctcctgatggcagatgtaaattgggatgt |
| F2 gene | ||||
| rs1799963 | G > A | 46761055 | 3′-UTR | F: gttcccaataaaagtgactctcagc R: agcctcaatgctcccagtgctattc |
| F5 gene | ||||
| rs6025 | T > C | 169519049 | Gln534Arg | F: ttacttcaaggacaaaatacctgtattcct R: gcctgtccagggatctgctcttacagatta |
| rs6027 | T > C | 169483561 | Asp2222Gly | F: gggtttttgaatgttcaattctagtaaata R: cacagccaaagagttccaggcgaagtgcaa |
| F7 gene | ||||
| rs6046 | G > A | 113773159 | Arg412Gln/Pro/Leu | F: acagtggaggcccacatgccacccactacc R: gggcacgtggtacctgacgggcatcgtcag |
| ITGB3 gene | ||||
| rs5918 | T > C | 45360730 | Leu59Pro | F: tttgggctcctgacttacaggccctgcctc R: gggctcacctcgctgtgacctgaaggagaa |
IL: interleukin; TNF: tumor necrosis factor; CRP: C-reactive protein; APO: apolipoprotein; LIPC: hepatic lipase; LPA: lipoprotein (a); VDR: vitamin D receptor; CASR: calcium-sensing receptor; OPG: osteoprotegerin; CALCR: calcitonin receptor; ITGB: integrin beta.