Figure 2.

miR-103-2-5p and miR-585-5p decrease PARP-1 expression. HAECs (a, b) or HUVECs (c, d) were transfected with precursor mimics for miR-103-2-5p, miR-585-5p, or scrambled (Scr) control. After 48 hrs, cells were lysed for RNA and protein analysis. (a, c) miRNA and PARP1 mRNA levels were quantified by RT-qPCR analysis. (b, d) Lysates were analyzed by SDS-PAGE and immunoblotted with anti-PARP-1, anti-GAPDH, and anti-β-actin antibodies. Histograms represent the mean + SEM and from three independent experiments ^∗P < 0.05 and P < 0.01 by Student's t-test.