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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Nat Genet. 2017 May 1;49(6):887–894. doi: 10.1038/ng.3857

Figure 2. DIM-2-mediated RIP requires the SUV39 histone methyltransferase DIM-5.

Figure 2

a, A cartoon representation of the canonical heterochromatin pathway in N. crassa (ref. 15).

b, RIP mutation profiles of the 802-bp construct. Crosses X8{60} (top) and X9{60} (bottom).

c, RIP mutation profiles of the 802-bp construct. Crosses X10{60}, X11{60}, X12{60} and X13{60} (top to bottom). Only the right-most portion of the endogenous repeat copy and the entire 600-bp segment of the linker region were sequenced.

d, RIP mutation profiles of the 802-bp construct. Crosses (left to right) X14{24}, X15{24} and X16{48}.

e, RIP mutation profile of the 802-bp construct. Cross X17{48}.

f, RIP mutation profiles of the 802-bp construct. Crosses (left to right) X18{24}, X19{24} and X20{48}.

g, A fraction of “linker” mutations reports the relative activity of DIM-2-mediated RIP. Each fraction value corresponds to the number of mutations in the 600-bp segment of the linker region normalized by the total number of mutations found in this 600-bp segment and in the repeated sequences. Analyzed crosses (left to right): X1, X4, X17, X18, X19, X20. The difference between any two fraction values is evaluated for significance using the chi-squared homogeneity test (P-value is indicated above the line) and the Fisher’s exact test (P-value is indicated below the line) on the actual mutation counts. *** P ≤ 0.001, NS P > 0.05. The number of spores analyzed for each cross is provided in curly brackets. Strain and cross genotypes are provided in Supplementary Table 1 and 2, respectively.