Table 1.

Identities of the protein spots 1–20 depicted in figure 1, representing major differences in the normal human serum proteome following exposure to adult schistosomes for 1 hour at 37°C versus control. Yellow highlights proteins known or implicated as being involved in the complement system. The theoretical molecular weight (MW) of each protein is listed as is the observed MW (kDa); pI, theoretical isoelectric point. The Accession Designation for each identified protein is given. The protein “Identification Score” is calculated by Mascot software and the value in parentheses, the “Peptide Count”, indicates the number of matching peptides identified by mass spectrometry in each case. The Protein Score Confidence Interval (CI) is listed (%); scores above 95% are significant, a lower score may still represent the correct identification, but requires corroboration. The Ratio represents the difference in spot volume between schistosome-exposed serum (S) and control serum (C) as determined by DeCyder software (see Figure 2 for an example).

Spot No.	Protein Name	MW Theoretical	MW Observed	Accession Designation	pI	Identification Score (Peptide Count)	Protein Score (CI %)	Ratio (S/C)
1	Alpha-2-macroglobulin	163.2	>110	A2MG_HUMAN	6.03	439 (38)	100	−1.54
2	Complement C3 (α chain)	113.0	85	CO3_HUMAN	5.55	456 (36)	100	−1.73
3	Alpha-1-antichymotrypsin	47.6	64	AACT_HUMAN	5.33	261 (17)	100	1.68
4	Complement factor B (b fragment)	57.0	64	CFAB_HUMAN	7.27	589 (25)	100	4.5
5	Protein DDX26B	96.6	50	DX26B_HUMAN	8.89	47 (8)	62	2.39
6	Complement C3 (α′2 fragment)	39.5	47	CO3_HUMAN	4.79	611 (25)	100	2.7
7	Actin, cytoplasmic 2	41.8	47	ACTG_HUMAN	5.31	299 (9)	100	1.79
8	Actin, cytoplasmic 2	41.8	47	ACTG_HUMAN	5.31	243 (9)	100	1.67
9	Clusterin	52.5	46	CLUS_HUMAN	5.89	116 (11)	100	2.63
10	Clusterin	52.5	45	CLUS_HUMAN	5.89	101 (12)	100	2.15
11	Complement factor H-related protein 1	30.6	40	FHR2_HUMAN	6.00	73 (6)	100	−1.92
12	Complement C4 (α3 fragment)	27	36	CO4B_HUMAN	6.89	196 (16)	100	1.69
13	Complement C3 (α′3 fragment)	24	35	CO3_HUMAN	6.89	437 (22)	100	1.81
14	Ig kappa chain C region	11.6	32	IGKC_HUMAN	5.58	338 (5)	100	1.54
15	Serum albumin	69.3	31	ALBU_HUMAN	5.92	364 (16)	100	2.13
16	Serum amyloid A-4 protein	14.7	23	SAA4_HUMAN	9.17	99 (7)	100	4.61
17	Hemoglobin subunit beta	15.98	17	HBB_HUMAN	6.75	688 (13)	100	19.96
18	Serum amyloid A-4 protein	14.74	15.5	SAA4_HUMAN	9.17	193 (7)	100	1.54
19	Complement C3 (α fragment)	9	14	CO3_HUMAN	9.69	161 (13)	100	3.63
20	Complement C3 (α fragment)	9	13	CO3_HUMAN	9.69	227 (15)	100	4.74