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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: Biochim Biophys Acta. 2016 Dec 18;1859(9 Pt A):1445–1455. doi: 10.1016/j.bbamem.2016.12.007

Figure 5.

Figure 5

Verification of non-interacting BRET pair using bimolecular fluorescence complementation (BiFC). A) Fluorescence microscopy of HEK293 cells co-transfected β2- AR/N-YFP + β2-AR/C-YFP. Positive BiFC is indicated by green fluorescence in the left panel. The middle panel shows the differential interference contrast (DIC) image of the same cells and the right panel shows the merged image of the left and middle panels. B) Left panel: fluorescence image of cells co-transfected with β2-AR/N-YFP + 5-HT2C/C-YFP. Middle panel: DIC image of same cells. Right panel: merged image of the left and middle panels. C) Left panel: fluorescence image of cells co-transfected with 5-HT2C/N-YFP + 5-HT2C/C-YFP. Middle panel: DIC image of same cells. Right panel: merged image of the left and middle panels. Red scale bar = 10μm. Images are representative of entire fields of cells from two transfections.