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. 2017 Jan 13;16:292–301. doi: 10.1016/j.ebiom.2017.01.004

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© 2017 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 1 — Expression of glutaminase, glutamate transporters and glutamine transporters in corneal endothelium. (A) RT-PCR of human and mouse corneal endothelial tissue for glutaminase (GLS1, GLS2, GGT), glutamate dehydrogenase (GDH) and glutamate transporters (EAAT1-5); ACTB: beta actin. (B) Immunofluorescence staining of human cornea section showing GLS1, GLS2 and GGT expression in cornea endothelium; (C) RT-PCR of HCEC showing expression of GLS1, GLS2, GGT, EAAT1-3, ten glutamine transporters as well as SLC4A11. Glutamine transporters expressed: SLC1A5, Na⁺-glutamine/neutral amino acid antiport; SLC6A19, Na⁺-glutamine co-transport; SLC7A5, glutamine/large neutral amino acid antiport; SLC7A8, glutamine/small neutral amino acid antiport; SLC38A1, Na⁺-glutamine cotransport; SLC38A2, Na⁺-glutamine cotransport; SLC38A3, Na⁺-glutamine/H⁺ antiport; SLC38A5, Na⁺-glutamine/H⁺ antiport; SLC38A7, Na⁺-glutamine/H⁺ antiport; SLC38A8, Na⁺-Neutral Amino Acid Transporter. GAPDH: glyceraldehyde 3-phosphate dehydrogenase. (D) Immunofluorescence staining of GLS1 (upper) and GGT (lower) in HCEC.