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. 2017 Jan 13;16:292–301. doi: 10.1016/j.ebiom.2017.01.004

Fig. 3.

Fig. 3

Glutamine supplies energy for corneal endothelial pump function. (A) ATP levels in cultured HCEC in conditional media (n = 23 in each); (B) Central corneal thickness (CCT) is better maintained with glutamine-supplemented perfusion. Left: time course of CCT change. Right: linear slope comparison. (C) Corneal deswelling curves in various Ringers from cold stored corneas: bicarbonate rich Ringer with glucose and glutamine (BR Glc/Gln); bicarbonate rich Ringer with glucose (BR Glc/−); and bicarbonate rich Ringer with glutamine (BR −/Gln). Left: time course of CCT changes. Right: first-order exponential decay rate constant comparison between groups. (D) Rabbit corneal endothelium glucose and glutamine consumption rate. (E) Cold stored human cornea deswelling curves in various Ringers: BR Glc/Gln and BR Glc/−. Left: time course of CCT changes. Right: first-order exponential decay rate constant comparison between groups. (F) Human corneal endothelium glucose and glutamine consumption rate. Data are shown as mean ± s.e.m., **p < 0.01; ***p < 0.001.