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. 2017 Jan 11;16:275–283. doi: 10.1016/j.ebiom.2017.01.011

Fig. 2.

Fig. 2

Imprinting genes in human oocytes (mother) and lymphocytes (offspring). A: IGF2 expression in oocytes donated by women with hyperandrogenism (HA, n = 10) or without hyperandrogenism (control, n = 12) was detected by immunofluorescence. B and C: Effects of 10− 9 M DHT on IGF2 and DNMT3a expression in human oocytes donated by women without hyperandrogenism were examined by immunofluorescence (n = 5 for each group). D: Expression of imprinting genes in lymphocyte of offspring conceived by women with (HA, n = 80) or without hyperandrogenism (control, n = 146). E and F: Methylation status of IGF2 and GRB10 in lymphocyte of offspring. Methylation status of the IGF2 DMR2 and GRB10 DMR was analyzed by BSP (n = 3 per group). The methylation rates of individual CpG sites within the IGF2 DMR2 and GRB10 DMR were presented as a line graph. *p < 0.05, vs. Ctrl, **p < 0.01, vs. Ctrl, ***p < 0.001, vs. Ctrl.