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. 2017 Jan 11;16:275–283. doi: 10.1016/j.ebiom.2017.01.011

Fig. 4.

Fig. 4

Expression and methylation status of Igf2 in rat oocytes (F0) and pancreatic islets (F1). A: Schematic diagram showing transmission of epigenetic marks from germ cells (F0, oocytes) into somatic cells (F1, pancreatic islets cells). B: Expression of Igf2 mRNA in oocytes. C: Expression of Igf2 mRNA in offspring islets (embryonic day 20 (E20), 3 and 8 weeks old). D and E: Overall methylation status of Igf2 in F0 oocytes and F1 pancreatic islets. F and G: Methylation status in individual CpG sites of Igf2 in F0 oocytes and F1 pancreatic islets. Methylation status of the Igf2 DMR2 was analyzed by bisulfite sequencing (n = 3 per group). The methylation rates of individual CpG sites within the Igf2 DMR2 are presented as a line graph. H: Expression of Dnmt3a in rat oocyte treated with in-vitro DHT (10− 9 M) by immunofluorescence. Data are presented as means ± SE. *p < 0.05, vs. Ctrl, **p < 0.01, vs. Ctrl, ***p < 0.001, vs. Ctrl.