Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jan 13;16:212–223. doi: 10.1016/j.ebiom.2017.01.015

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Fig. 1 — Loss of MyoD facilitates the adipogenic transdifferentiation of C2C12 myoblasts. (a) Schematic diagram of MyoD partial protein coding region and the 2 targeting loci of MyoD sgRNA. The targeting loci are in red. (b) DNA sequence map around the targeting locus of the cleaved band amplified from myoblasts transfected with both gRNAs. (c) Immunostaining of MyoD (Red) and DAPI (Cyan) in control and MyoD^KO C2C12 myoblasts. (d) Representative images of ORO staining of C2C12 myoblasts after adipogenic differentiation. (e and f) qPCR analysis of Myog (e) and adipogenic markers genes (f). Error bars represent mean with SD of five independent biological replicates.^⁎P < 0.05 (Student's t-test).