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. 2017 Jan 13;16:212–223. doi: 10.1016/j.ebiom.2017.01.015

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© 2017 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 5 — Overexpression of MyoD impairs brown adipogenesis of wildtype BAT SVF cells. (a) ORO staining of brown adipocytes differentiated from wildtype BAT SVF cells transduced with control or MyoD^OE adenovirus. (b and c) Immunostaining of BODIPY (red) in control (b) and MyoD^OE (c) brown adipocytes (d) The ratio of BODIPY signal in GFP⁺ cells in control and MyoD^OE brown adipocytes. Five different areas analyzed in each experiment. (e–g) qPCR analysis of myogenic markers (e) miRNAs (f) and adipogenic markers (g). Error bars represent mean with SD of three independent biological replicates.^⁎P < 0.05 (Student's t-test).